- GLLP Brochure
- Laboratory Leadership Competency Framework (WHO, 2019)
- GLLP Frequently Asked Questions (WHO, 2022)
- GLLP at a Glance (WHO, 2021)
- Critical gaps in laboratory leadership to meet global health security goals (WHO, 2017)
- Supporting the Development of Strong Laboratory Leaders for Global Health Security (2019)
Global Health Resources
​​​APHL publishes laboratory practice guides, training manuals and materials to support development of laboratory plans, policies and infrastructure.
Contact the Global Health team: [email protected]
About Our Work
Technical Resources
Laboratory Data Systems
Overview of the Open Laboratory Data Repository platform, for ministries and country-level public health systems
Overview of the Open Laboratory Data Repository platform's functional components, for software developers, engineers and IT administrators
This guide follows the sequence of an international LIS implementation. Users can begin with the section that corresponds to their place in the LIS implementation cycle.
A tool to provide laboratories with questions to discuss when evaluating data quality and data use of the laboratory information system (LIS).
A means for laboratories to verify that training and LIS implementation have been appropriately completed.
A tool to assign roles and responsibilities for completing tasks related to the laboratory information system (LIS).
A framework for a laboratory to craft their own steps and activities that need to be carried out for the laboratory information system (LIS) to succeed.
This guide explores the utility and opportunities available with integrated laboratory information systems (LIS), for both individual laboratories and national-level public health systems.
High level summary of global public health data exchange solutions.
A guide for laboratories around the world to design a road map for establishing a data exchange platform that meets your circumstances and needs.
Una guía que los laboratorios de todo el mundo diseñen un proceso para establecer una plataforma de intercambio de datos que se ajuste a sus circunstancias y necesidades
A framework of tiered positions and roles that work with and support the laboratory information system (LIS).
A tool to monitor and assess the day-to-day functioning of the laboratory information system (LIS).
This document provides guidelines to laboratory managers on how to craft and implement a detailed data backup and recovery plan and carry out regular backups of data and applications.
A tool to evaluate data quality and turn around time within the laboratory information system (LIS).
A set of questions to evaluate steps taken to ensure ownership of the laboratory information system (LIS) and assess laboratory and national policies to support effective utilization of the LIS.
Select findings from the 2019 APHL All-Hazards Laboratory Preparedness Survey highlight the importance of public-private partnerships in responding to public health threats.
Learn more about how we're improving antimicrobial resistance data collection and reporting in Zambia.
Learn how we're improving influenza data integration practices in Kenya to enhance surveillance efforts.
Learn how to protect laboratory data and ensure data confidentiality, integrity and availability around the world.
Sequencing and Bioinformatics
OXFORD NANOPORE TECHNOLOGIES: MIDNIGHT PROTOCOL
THERMOFISHER PLATFORMS: AMPLISEQ PROTOCOL
Illumina
COVIDSeq Protocols
- Library Prep (SARS-CoV-2) (Publisher: Illumina; April 2021)
- MiSeq Workbook
- Sample Sheet
ARTIC Protocols
- Library prep (HIV 1/2 sequencing) (Publisher: OR State Public Health Laboratory & APHL; April 2022)
MiSeq Sequencer Documents
COVIDSeq Protocols
- Library prep (SARS-CoV-2)
- iSeq workbook
- Sample Sheet
ARTIC Protocols
- Library prep (HIV 1/2 sequencing) (Publisher: OR State Public Health Laboratory & APHL; April 2022)
Sequencer Documents
COVIDSeq Protocols
- Library Prep (SARS-CoV-2) (Publisher: Illumina; April 2021)
- Workbook and Sample Sheet
Artic Protocols
Sequencer Documents
COVIDSeq Protocols
- Library Prep (SARS-CoV-2)
Food Safety - PulseNet International
Training Series
Journal Articles
Standard Operating Procedures
APHL, in collaboration with CDC, is committed to assisting all PulseNet participating laboratories (USA and International) in achieving the highest quality of data before sharing sequencing results with their respective countries and/or regions. Part of that commitment consists of the development and implementation of standardized laboratory and analysis protocols that enable the exchange and comparison of whole genome sequence data between laboratories.
The PulseNet Standard Operating Procedures (SOPs) provided below were established specifically for the PulseNet USA laboratories, with the understanding that most of the PulseNet USA standards will apply, within varying degrees, to our PulseNet International partners. We encourage PulseNet International laboratories to adopt these standards whenever possible and to modify them in a manner consistent with their internal policies or guidelines established by their institution, state, or country.
For questions concerning PulseNet International SOPs, please contact [email protected].
PND05 Analyzing Sequences Using PulseNet 2.0 v4
PNL33 DNA Extraction and Quality
PNL34.W1 Nextera XT Library Prep Workbook for MiSeq v7
PNL35.W1 Illumnia DNA Prep Workbook
PNL37 Kapa Hyperplus Library Prep
PNL42 NEBNext Ultra II FS Library Prep
PNL44 PulseNet Rapid Library Prep SOP
PNQ09 WGS Proficiency Testing v9
PNL34.W1 Nextera XT Library Prep Workbook for MiSeq v6.1
PNL35.JA1 Illumina DNA Prep Quick Guide v3
PNL35.W1 Illumina DNA Prep Workbook v17
PNL35.W3 Illumina DNA Prep Checklist v8
PNL35.W4 Illumina DNA Prep Index Tracking v5
PNL36.W1- PN QIAseq FX Library Prep Workbook
PNL36-1 Appendix PN QIAseq FX Indices Tracking Worksheet
PNL37.W1- PN KAPA HyperPlus Library Prep Workbook
PNL37-1 Appendix PN KAPA HyperPlus Indices Tracking Worksheet
PNL41.W1 Ion Torrent S5 Workbook
PNL42.W1_NEBNext_Ultra_II_FS_Workbook v1
PNL44.W1 PulseNet Rapid Prep Workbook v1
PNL44.W2 PulseNet Rapid Library Prep Checklist v1
Training and Professional Development
For a complete list of our offerings, visit: